鸡L-FABP启动子区C/EBPα结合位点的定点突变分析

doi:10.11843/j.issn.0366-6964.2015.09.003

Abstract

Abstract:

In order to further determine the C/EBPα binding site of L-FABP and analyze its role in the regulation of L-FABP transcription，the C/EBPα binding site in L-FABP promoter was effectively mutated using site-directed mutagenesis in the current study.The results showed that the expression of L-FABP was significantly repressed by C/EBPα，but the L-FABP promoter activity was significantly promoted after the mutation of C/EBPα binding site.In a conclusion，the expression of L-FABP was significantly repressed by C/EBPα，and C/EBPα probably involved in the regulation of L-FABP expression through this binding site.These results will provide a foundation for further research on the regulatory mechanism and function of chicken C/EBPα on L-FABP gene.

CLC Number:

S831.2

HE Qi，SUN Ying-ning，LI Hui，WANG Qi-gui. Site Directed Mutagenesis for C/EBPα Binding Sites in Chicken L-FABP Promoter[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1496-1501.

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References

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Site Directed Mutagenesis for C/EBPα Binding Sites in Chicken L-FABP Promoter

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